A comparison of 33 HI2:ST3 plasmids demonstrated that those of Australian origin have not acquired resistances to extended-spectrum beta-lactams, colistin, fosfomycin or rifampicin, unlike those originating from Asia. Plasmid comparisons showed that pF2_18C_FIB shared homology with EHEC virulence plasmids such as pO103 while pF2_18C_HI2 is a large multidrug resistance IncHI2:ST3 plasmid. coli ST302 strains carry diverse VAGs, including those that typically associated with extraintestinal pathogenic E. coli ST302 strains and short-read gene screens were used to compare their virulence-associated gene (VAG) and antimicrobial resistance gene (ARG) cargo. Public databases were interrogated for WGSs of E. coli ST302 strains in proximity to EHEC ST32 (O145:H28) strains. Through WGS and hybrid assembly we present the first closed chromosome, and two circularized plasmids of an ST302 strain - F2_18C, isolated from a healthy pig in Australia. Escherichia coli ST302 (O108:H9, O182:H9, O45:H9) are aEPEC that have been isolated from diarrheic human, pig and rabbit hosts, as well as in healthy pigs, however, no study to date has focused on E. While aEPEC can cause disease outright, the potential to acquire stx, one of the most potent bacterial toxins known, merits close monitoring. However, phylogenetic analyses often associate aEPEC with EHEC, promoting the hypothesis that aEPEC are the progenitors of EHEC, which is supported by aEPEC conversion to EHEC by stx-carrying phages.

They are differentiated from typical EPEC by the lack of bundle-forming pili, and from EHEC by the lack of phage-mediated stx toxins.

3NSW Department of Primary Industries, Elizabeth MacArthur Agricultural Institute, Menangle, NSW, AustraliaĪEPEC are associated with persistent diarrhea, and diarrheal outbreaks in both humans and animals worldwide.

2Australian Centre for Genomic Epidemiological Microbiology, University of Technology Sydney, Sydney, NSW, Australia.1ithree institute, University of Technology Sydney, Sydney, NSW, Australia.Sticky ends from different DraIII sites may not be compatible.ĮcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.Ĭ T G A A G ( N ) 14 N N G A C T T C ( N ) 14Ĭleavage may be enhanced when more than one copy of the AcuI recognition sequence is present.Veronica M. Sticky ends from different TaqII sites may not be compatible. G A C C G A ( N ) 9 N N C T G G C T ( N ) 9

Sticky ends from different BciVI sites may not be compatible.īssHII is typically used at 50☌, but is 75% active at 37☌.īclI is typically used at 50-55☌, but is 50% active at 37☌.

Snapgene e coli